This page contains a list of the solution NMR experiments most commonly used in protein NMR assignment and structure calculation. For each experiment there is an illustration showing which atoms are observed (pink) and through which atoms magnetisation flows (light blue). An example spectrum shows what it should look like and particular features and considerations are highlighted. The descriptions here are very simplistic and for a full description, including pulse sequences and product operator treatment, the reader should consult the original papers or a book such as Protein NMR Spectroscopy – Principles and Practice by J. Cavanagh, W. Fairbrother, A.G. Palmer III and N.J. Skleton (Academic Press) (Link to Publisher).
Solid-state NMR spectroscopists may be interested in my recentarticle on ‘Solid-state MAS NMR resonance assignment methods for proteins’published in Progress in NMR Spectroscopy (free download until 27 July 2018here).
- Solution NMR
- Solid-State MAS NMR
- General NMR
- CCPNmr Analysis