15N-NOESY-HSQC

References:
D. Marion, P.C. Driscoll, L.E. Kay, P.T. Wingfield, A. Bax, A.M. Gronenborn and G.M. Clore (1989) Biochemistry 28 6150-6156. (Link to Article)
D. Marion, L.E. Kay, S.W. Sparks, D.A. Torchia and A. Bax (1989) J. Am. Chem. Soc. 111 1515-1517. (Link to Article)
E.R.P. Zuiderweg and S.W. Fesik (1989) Biochemistry 28 2387-2391. (Link to Article)

Minimum labelling: 15N

Dimensions: 3

To start with, magnetisation is exchanged between all hydrogens using the NOE. Then the magnetisation is transferred to neighbouring 15N nuclei and back to 1H for detection.

15N-NOESY-HSQC magnetisation transfer

This spectrum can be used to obtain restraints for structure calculations. In this case the NOESY mixing time should probably be around 80ms. It can also be used to help assignment, and for small to medium-sized proteins, assignment using this and 15N-TOCSY-HSQC only is possible. In this case it may be useful to use a slightly longer NOESY mixing time.

15N-NOESY-HSQC spectrum